Info derived from imdb. Only works 2013 and later being tracked. Only actors in 5+ non-archival episodes noted.
Buddy Ebsen (Jed Clampett): died in 2003.
Irene Ryan (Daisy Moses): died in 1973.
Donna Douglas (Elly May Clampett): died in 2015; in Chronicles of Life Trials (2013).
Max Baer Jr. (Jethro Bodine): 1991, as Self in 2009.
Raymond Bailey (Milburn Drysdale): died in 1980.
Nancy Kulp (Jane Hathaway): died in 1991.
Harriet E. MacGibbon (Mrs. Margaret Drysdale): died in 1987.
Bea Benaderet (Cousin Pearl Bodine): died in 1968.
Shug Fisher (Shorty Kellems) died in 1984.
Danielle Mardi (Helen Thompson): no other known acting roles.
Linda Henning (Jethrine Bodine): last known acting role in 2007, as Self in 2009.
Sharon Tate (Janet Trego) died in 1969.
Frank Wilcox (John Brewster): died in 1974.
Elvia Allman (Elverna Bradshaw): died in 1992.
Roger Torrey (Mark Templeton); died in 1985.
Larry Pennell (Dash Riprock): died in 2013.
Frank Cady (Sam Drucker): died in 2012.
Judy Jordan (Babs/Patricia Switzer): died in 2001.
Bill Baldwin (Announcer): died in 1982.
Arthur Gould-Porter (Ravenswood): died in 1987.
Mike Minor (Steve Elliott): died in 2016.
Milton Frome (Lawrence Chapman): died in 1989.
Ray Kellogg (Police Officer/Gate Guard): died in 1981.
Bettina Brenna (Miss Gloria Buckles): only known subsequent acting role in 1996.
Lester Flatt (Himself): died in 1979.
Earl Scruggs (Himself): died in 2012; as Self: Béla Fleck: How To Write A Banjo Concerto (2014).
Charles Lane (Foster Phinney): died in 2007.
Richard Caldicot (John Faversham): died in 1995.
Phil Gordon (Jasper 'Jazzbo' Depew): died in 2010; as Production Accountant: Independent Lens (1 episode 2013).
Lori Saunders (Elizabeth Gordon/ Bobbie Jo Bradley): last known acting role in 1980, as Self in 2005.
Venita Wolf (Miss Murray): died in 2014.
Phil Silvers (Shifty Shafer aka Honest John): died in 1985.
Kathleen Freeman (Flo Shafer): died in 2001.
Percy Helton (Homer Cratchit): died in 1971.
Lloyd 'Shad' Heller (Shad): died in 1991.
Roy Roberts (John Cushing): died in 1975.
Paul De Rolf (various): no known subsequent acting roles, as Choreographer in 1985.
Fred Clark (Dr. Roy Clyburn): died in 1968.
Alan Reed Jr. (Sheldon Epps): last known acting role in 1969.
Richard Deacon (Dr. Klingner): died in 1984.
Joi Lansing (Gladys Flatt): died in 1972.
Jerry Brutsche (various): last known acting role in 1993, as Stunt Performer in 2002, as Still Photographer in 2005.
Barney Elmore (Chauffeur Parkins): last known acting role in 1971.
Jeannie Bell (Sugar Jean Bell): last known acting role in 1977.
Jack Bannon (various): in Without a Ladder (2013); Mistrust (2016) in post-production.
Helen Ko (various): last known acting role in 1976.